As PRF protocols are constantly updated, it is common to have to create new settings on the Horizontal Centrifuge. Luckily, this is a very easy process. Please watch the video below which provides instructions for creating custom settings.
You cannot simply use the RPM from a specific study or centrifuge will not work, as the RPM is not the critical factor to consider when setting the machine for a specific PRF protocol. The important number to use is the g-force or RCF.
RCF (RCF max) describes the amount of accelerative force applied to a sample in a centrifuge. As RCF is a function of the RPM and radius of the centrifuge, PRF protocol results derived from using centrifuges with different radii are not comparable, and the RPM may need to be adjusted to achieve a similar RCF on a different centrifuge. However, different PRF protocols can be replicated assuming you get the correct information about the protocol and the machine used.
So, if you want to use another protocol that you read about in the literature or heard at a lecture, you should inquire from the any presenter what particular centrifuge they are using for a particular protocol, and what RPM/time they have used, as well as the G-Force (defined as RCFmax) . With the Horizon 6 Flex Horizontal Centrifuge, you can create PRF as described in any protocol by properly setting the RPM/time which would correspond to the proper RCF.
There is a simple online calculator to figure out the equation for RCF (we assume RCFmax is used, which is the international standard of measurement). The radius on the Horizon 6 Flex is approximately 127mm. In addition, the Horizonal Centrifuge from DDSGadget also flashes the RCF value when you set the RPM, so it is easy to see what RCF is equivalent for a specific RPM.
